Topic 151

proteins complexes membrane protein proximity biology biotinylation interaction proteome partners cell assembly interactions binding plasma adaptor bioid roles labeling complex cellular interactome endocytosis formation molecular biochemical spectrometry functional secretory tplate purification interact identified live interacting endocytic tagged family affinity terminal recruitment localization dependent mass pathway structural domain via adaptors biochemistry trafficking function essential crispr tpc conserved biotin components peroxidase directly mammalian interactors proteomics assemble turboid vesicles here surface regulate interface situ apex2 clathrin remodeling reveals fusion ateh pan1 interestingly target machinery regulators membranes vesicle native previously sufficient recruited mediated cargo identify transmembrane critical novel approach bait interactomes pore molecule biotinylated

70 items. Top items listed below.

The TPLATE subunit is essential for structural assembly of the endocytic TSET complex 151 34 8 3

CBRPP: a new RNA-centric method to study RNA-protein interactions 151 45 33 3

In vivo discovery of RNA proximal proteins in human cells via proximity-dependent biotinylation 151 49 33 3

Establishment of Proximity-dependent Biotinylation Approaches in Different Plant Model Systems 151 45 8 4

Identifying cardiac actinin interactomes reveals sarcomere crosstalk with RNA-binding proteins 151 95 49 3

Wnt-inducible Lrp6-APEX2 Interacting Proteins identify ESCRT Machinery and Trk-Fused Gene as Components of the Wnt Signaling Pathway 151 34 3

Clathrin senses membrane curvature 151 34 8

Distinct EH domains of the endocytic TPLATE complex confer lipid and protein binding. 151 8 3

Mapping Functional Protein Neighborhoods in the Mouse Brain 151 8

A SARS-CoV-2 - host proximity interactome 151 41 8

On-rate modulation of cadherin interactions by chemical fragments 151 63 8 3

RNA-protein interaction mapping via MS2 or Cas13-based APEX targeting 151 33

Proximity-dependent proteomics reveals extensive interactions of Protocadherin-19 with regulators of Rho GTPases and the microtubule cytoskeleton 151 34 3

Heh2/Man1 may be an evolutionarily conserved sensor of NPC assembly state 151 34 8 3

Enzymatic RNA Biotinylation for Affinity Purification and Identification of RNA-protein Interactions 196 151 45 8 3

High temporal resolution reveals simultaneous plasma membrane recruitment of the TPLATE complex subunits. 151 34 16 8 3

Nanobody-dependent delocalization of endocytic machinery in Arabidopsis root cells dampens their internalization capacity 151 16 3

Regulated assembly of the ER membrane protein complex 151 34 8 3

A high-density human mitochondrial proximity interaction network. 151 34 8

RNP-MaP: In-cell analysis of protein interaction networks defines functional hubs in RNA 151 8

ACSL3 is a novel GABARAPL2 interactor that links ufmylation and lipid droplet biogenesis 151 34 33 3

Golgi SM protein Sly1 promotes productive trans-SNARE complex assembly through multiple mechanisms 151 34 8 3

Origin of the nuclear proteome on the basis of pre-existing nuclear localization signals in prokaryotic proteins 151 34 8 3

In situ detection of protein interactions for recombinant therapeutic enzymes 151 149 129 8 3

In cell architecture of the nuclear pore complex and snapshots of its turnover 151 8 3

Computational and experimental characterization of the novel ECM glycoprotein SNED1 and prediction of its interactome 151 8 3

Mapping transmembrane binding partners for E-cadherin ectodomains. 151 34 8 3

Cargo-specific recruitment in clathrin and dynamin-independent endocytosis 151 34 3

Structural determination of Streptococcus pyogenes M1 protein interactions with human immunoglobulin G using integrative structural biology 151 8 3

Arabidopsis Myosin XIK Interacts with the Exocyst Complex to Facilitate Vesicle Tethering during Exocytosis 151 34 16 8 3

APEX2 proximity proteomics resolves flagellum subdomains and identifies flagellum tip-specific proteins in Trypanosoma brucei 151

Global BioID-based SARS-CoV-2 proteins proximal interactome unveils novel ties between viral polypeptides and host factors involved in multiple COVID19-associated mechanisms 151 1

Endosidin20 targets cellulose synthase catalytic domain to inhibit cellulose biosynthesis 151 8 3

Driving Integrative Structural Modeling with Serial Capture Affinity Purification 151 45 8

Mitotic checkpoint protein Mad1 is required for early Nup153 recruitment to chromatin and nuclear envelope integrity 151 34 8 3

Cell type-selective secretome profiling in vivo 151 3

Functional domain studies uncover novel roles for the ZTL Kelch repeat domain in clock function 151 63 8 3

Cardiolipin targets a dynamin related protein to the nuclear membrane 151 34 8 3

Ancient MAPK ERK7 is regulated by an unusual inhibitory scaffold required for Toxoplasma apical complex biogenesis 34 8 3

Dual Proteome-scale Networks Reveal Cell-specific Remodeling of the Human Interactome 151 45 8 3

Rationalizing PROTAC-mediated ternary complex formation using Rosetta 151 8

Comparative multiplexed interactomics of SARS-CoV-2 and homologous coronavirus non-structural proteins identifies unique and shared host-cell dependencies 1

A Myosin-7B dependent endocytosis pathway mediates cellular entry of α-Synuclein fibrils and polycation-bearing cargos 151 34 3

Definition of the fibroblast adhesome using multiplexed proximity biotinylation 151 55

BPIFB3 interacts with ARFGAP1 and TMED9 to regulate non-canonical autophagy and RNA virus infection 41 34 3

An in vitro vesicle formation assay to analyze protein sorting in the secretory transport pathway 151 34 8 3

PKA-mediated phosphorylation of SPG11/spatacsin regulates binding with a subset of 14-3-3 proteins 151 34 3

An interaction network of RNA-binding proteins involved in Drosophila oogenesis 49 45 34 9 8 3

A conformational switch driven by phosphorylation regulates Ykt6 activity in macroautophagy 34 8 3

A structural model of a Ras-Raf signalosome 151 8 3

The guidance and adhesion protein FLRT2 dimerizes in cis via dual Small-X3-Small transmembrane motifs 151 8 3

Crosstalk between chloroplast protein import and the SUMO system revealed through genetic and molecular investigation 151 3

State-dependent protein-lipid interactions of a pentameric ligand-gated ion channel in a neuronal membrane 163 91 82 8 3

EPH tyrosine kinase receptors regulate epithelial morphogenesis and phosphorylate the PAR-3 scaffold protein to modulate downstream signaling networks 102 34 3

HIV-1 Gag protein with or without p6 specifically dimerizes on the viral RNA packaging signal 8 3

Identification of a new regulation pathway of EGFR and E-cadherin dynamics 102 34 3

A SARS-CoV-2 BioID-based virus-host membrane protein interactome and virus peptide compendium: new proteomics resources for COVID-19 research 45 1

Mapping proteolytic neo-N termini at the surface of living cells 151 18 8

Gatekeeper helix activates Golgi SM protein Sly1 and directly mediates close-range vesicle tethering 151 34 8 3

The TRIM9/TRIM67 neuronal interactome reveals novel activators of morphogenesis 151 11 3

Proteomic profiling of mitochondrial-derived vesicles in brain reveals enrichment of respiratory complex sub-assemblies and small TIM chaperones 95 34 3

Flagellar targeting of an arginine kinase requires the conserved Lipidated Intraflagellar Transport (LIFT) pathway in Trypanosoma brucei 151 34 3

Structure of MlaFB uncovers novel mechanisms of ABC transporter regulation 34 8 3

The nanoscale anatomy of exocytic dense-core vesicles in neuroendocrine cells 151 8


Functional partitioning of a liquid-like organelle during assembly of axonemal dyneins 151 34 8 3

A Glu-Glu-Tyr sequence in the cytoplasmic tail of the M2 protein renders IAV susceptible to restriction of HA-M2 association in primary human macrophages 41 34 8 3

Co-expression signatures of combinatorial gene regulation 134 31 9 3

UNC-10/SYD-2 complex is sufficient to link kinesin-3 UNC-104 to RAB-3 containing synaptic vesicles in the absence of the motor's PH domain 151 34 8 3

SurA is a "Groove-y" Chaperone That Expands Unfolded Outer Membrane Proteins 8

A cross-nearest neighbor/Monte Carlo algorithm for single molecule localization microscopy defines interactions between p53, Mdm2, and MEG3 151 18 8

Towards the molecular architecture of the peroxisomal receptor docking complex 8 3

Identification of a PGXPP degron motif in dishevelled and structural basis for its binding to the E3 ligase KLHL12 63 34 8 3

Ndel1 promotes keratin assembly and desmosome stability 34 3

A Highly Conserved 310-Helix Within the Kinesin Motor Domain is Critical for Kinesin Function and Human Health. 151 63 8 3

Detecting nanoscale distribution of protein pairs by proximity dependent super-resolution microscopy 70 18 8 4

Systematic discovery and functional interrogation of SARS-CoV-2 viral RNA-host protein interactions during infection 103 1

Dynamic tracking and identification of tissue-specific secretory proteins in the circulation of live mice 151 18 3

APEX2 proximity biotinylation reveals protein dynamics triggered by B cell receptor activation 88 27 8 3

Identifying Cancer-Relevant Mutations in the DLC START Domain using Evolutionary and Structure-Function Analyses 142 63 3

Spectrin Interactome under normal and HbE-disease conditions 151 3

A G protein-coupled receptor-like module regulates Cellulose Synthase secretion from the endomembrane system in Arabidopsis 34 16 3

SECAT: Quantifying differential protein-protein interaction states by network-centric analysis 45 38 4

Split-TurboID enables contact-dependent proximity labeling in cells 69 34 8 3

The electrostatic allostery could be the trigger for the changes in dynamics for the PDZ domain of PICK1 63 8 3

A regulatory interface on RIPK2 is required for XIAP binding and NOD signaling activity 102 34 3

TMEM70 forms oligomeric scaffolds within mitochondrial cristae promoting in situ assembly of mammalian ATP synthase proton channel 8 3

The Flemmingsome reveals an ESCRT-to-membrane coupling required for completion of cytokinesis 151 34 8 3

Heimdallarchaea encodes profilin with eukaryotic-like actin regulation and polyproline binding 151 55 34 8 3

BioID based proteomic analysis of the Bid interactome identifies novel proteins involved in cell cycle dependent apoptotic priming. 102 34 3

Proximity-based proteomics reveals the thylakoid lumen proteome in the cyanobacterium Synechococcus sp. PCC 7002 45 8

Structure of the complete, membrane-assembled COPII coat reveals a complex interaction network. 69 51 34 8 3

Pinpointing of cysteine oxidation sites in vivo by high-resolution proteomics reveals mechanism of redox-dependent inhibition of STING 45 3

Nucleotide binding, evolutionary insights and interaction partners of the pseudokinase Unc-51-like kinase 4 63 8 3

The low complexity regions in the C-terminus are essential for the subcellular localisation of Leishmania casein kinase 1 but not for its activity 34 3

Structural and dynamic characterization of the C-terminal tail of ErbB2: disordered but not random 149 63 8 3

Gene co-expression analysis of human RNASEH2A reveals functional networks associated with DNA replication, DNA damage response, and cell cycle regulation 120 9 3

Uptake of exogenous serine is important to maintain sphingolipid homeostasis in Saccharomyces cerevisiae 69 34 3

The insulin receptor adaptor IRS2 is an APC/C substrate that promotes cell cycle protein expression and a robust spindle assembly checkpoint 102 34 3

Molecular basis for substrate recruitment to the PRMT5 methylosome 151 136 108 34 3