Topic 70
microscopy imaging resolution fluorescence optical illumination super light 3d image microscope sheet biophysics images volumetric fluorescent live diffraction enables demonstrate microscopes techniques photon technique optics conventional high bioengineering field speed localization confocal spatial nm sectioning tissue molecule scanning reconstruction sample biological single method cellular limited excitation fast deep refractive laser allows smlm volume imaged present nanoscale structures without sim depth fluorophores dimensional limit clearing detection structured sub photobleaching large paint plane applications fluorophore aberrations acquisition versatile scattering view fixed introduce free brain phototoxicity axial widefield samples electron subcellular visualization ultrastructure label tissues capability use achieve beam methods volumes cleared here
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