Topic 33

crispr cas9 editing genome engineering gene target dna targeted targeting cas efficiency efficient synthetic guide system genetic sgrna applications rna systems genes genetics grna screening developed repair mediated tools screens technology engineered library biology directed grnas mutations edited based off rnas lines delivery platform nuclease sequences interference reporter knockout precise endogenous tool deletions single strategy generation double genomic expression cleavage throughput strand demonstrate hdr homology nucleases powerful sgrnas promoter dcas9 knock programmable efficiently generated design generate versatile used cas12a base will desired inducible plasmid vector coli recombination endonuclease screen enables robust efficiencies mutagenesis breaks edits homologous method vivo guided approach

479 items. Top items listed below.

A viral guide RNA delivery system for CRISPR-based transcriptional activation and heritable targeted DNA demethylation in Arabidopsis thaliana 33 16

Perturbing proteomes at single residue resolution using base editing 139 33 9 4

Type III-A CRISPR systems as a versatile gene knockdown technology 33 9

Resources for genome editing in livestock: Cas9-expressing chickens and pigs 33 9

Multiplexed conditional genome editing with Cas12a in Drosophila 33 18

Efficient Cas9 multiplex editing using unspaced gRNA arrays engineering in a Potato virus X vector 33 7

Massively parallel quantification of CRISPR editing in cells by TRAP-seq enables better design of Cas9, ABE, CBE gRNAs of high efficiency and accuracy 33 4

A Pipeline for Precise and Efficient Genome Editing by sgRNA-Cas9 RNPs in Drosophila 33 4

Efficient Gene Targeting in Maize using Inducible CRISPR-Cas9 and Marker-Free Donor Template 33 16

A CRISPR/Cas9 vector system for neutrophil-specific gene disruption in zebrafish 33 3

Enhancing CRISPR deletion via pharmacological delay of DNA-PK 33 3

Generating single-sex litters: development of CRISPR-Cas9 genetic tools to produce all-male offspring 33

Programmable RNA Targeting using CasRx in Flies 33

A functional screening platform for engineering chimeric antigen receptors with reduced on-target, off-tumour activation 33 14 10 3

Evaluation of CRISPR gene-editing tools in zebrafish identifies spurious mutations in 'mock control embryos 33 9 4

Addition of Multiple Introns to a Cas9 Gene Results in Dramatic Improvement in Efficiency for Generation of Gene Knockouts in Plants 159 33 16 9

CAS-LiveFISH: Simple and versatile imaging of genomic loci in live mammalian cells and early pre-implantation embryos 33 18 8

A novel prokaryotic CRISPR-Cas12a based tool for programmable transcriptional activation and repression 33 12

CRISPR-induced indels and base editing using the Staphylococcus aureus Cas9 in potato 33 4

Engineering an RNA-based tissue-specific platform for genetic editing through use of a miRNA-enabled Cas12a 33 18

Targeted genome editing in Nicotiana tabacum using inducible CRISPR/Cas9 system 33 16 9

In vitro and In vivo Genetic Disease Modelling via NHEJ precise deletions using CRISPR/Cas9 33

CRISPR-finder: A high throughput and cost effective method for identifying successfully edited A. thaliana individuals 7 4

Efficient immune cell genome engineering with improved CRISPR editing tools 33 18

A simple and highly efficient method for multi-allelic CRISPR-Cas9 editing in primary cell cultures 33 7 4

Evaluation of Mosaicism and Off Target Mutations in CRISPR-Mediated Genome Edited Bovine Embryos 33

CRISPR/Cpf1 mediated genome editing enhances Bombyx mori resistance to BmNPV 33 12

Efficient Development of Platform Cell Lines Using CRISPR-Cas9 and Transcriptomics Analysis 33 9

Targeted Genome Editing of Bacteria Within Microbial Communities 33 29 12 5

High-throughput screens of PAM-flexible Cas9 variants for gene knock-out and transcriptional modulation 33

Efficient replacement of long DNA fragments via non-homologous end joining at non-doding regions 33

Frequent loss-of-heterozygosity in CRISPR-Cas9-edited early human embryos 33 9

Enhancement of Target Specificity of CRISPR-Cas12a by Using a Chimeric DNA-RNA Guide 33

Amplification-free long read sequencing reveals unforeseen CRISPR-Cas9 off-target activity 25 4

gEL DNA, a cloning- and PCR-free method for CRISPR-based multiplexed genome editing 33 7 4

Self-cutting and integrating CRISPR plasmids (SCIPs) enable targeted genomic integration of large genetic payloads for rapid cell engineering 33 18

A genome-scale CRISPR interference guide library enables comprehensive phenotypic profiling in yeast 139 33 9

A Homology Independent Sequence Replacement Strategy in Human Cells Using a CRISPR Nuclease 33

Cas9 fusions for precision in vivo editing 33 18

A selectable, plasmid-based system to generate CRISPR/Cas9 gene edited and knock-in mosquito cell lines 48 33

Reprogramming the endogenous type III-A CRISPR-Cas system for genome editing, RNA interference and CRISPRi screening in Mycobacterium tuberculosis 33 12 9

MAGIC: Mosaic Analysis by gRNA-Induced Crossing-over 7 4

High-throughput in vitro specificity profiling of natural and high-fidelity CRISPR-Cas9 variants 33

CRISPR-nRAGE, a Cas9 nickase-reverse transcriptase assisted versatile genetic engineering toolkit for E. coli 33 18

Genome editing in mammals using CRISPR type I-D nuclease 33

Highly efficient CRISPR-Cas9-mediated gene knockout in primary human B cells for functional genetic studies of Epstein-Barr virus infection 33 3

CRISPR/Cas9-mediated gene editing induces neurological recovery in an A53T-SNCA overexpression rat model of Parkinson's disease 54 33 10 3

Generation and Validation of Versatile Inducible CRISPRi Embryonic Stem Cell and Mouse Models 33 20 9 3

Modulation of metabolic functions through Cas13d-mediated gene knockdown in liver 33 10 3

Harnessing CRISPR-Cas9 for genome editing in Streptococcus pneumoniae 33 12

An Efficient Vector-based CRISPR/Cas9 System in an Oreochromis mossambicus Cell Line using Endogenous Promoters 33

The inducible lac operator-repressor system is functional in zebrafish cells 33 20 3

Adapting CRISPR/Cas9 System for Targeting Mitochondrial Genome 33 3

One-shot generation of duodecuple (12x) mutant Arabidopsis: Highly efficient routine editing in model species 33 16

Upgraded CRISPR/Cas9 Tools for Tissue-Specific Mutagenesis in Drosophila 7 4

Validation and Long-Term Follow Up of CD33 Off-Targets Predicted In Vitro and In Silico Using Error-Corrected Sequencing in Rhesus Macaques 7 4

CRISPR/Cas "non-target" sites inhibit on-target cutting rates 33

Peel-1 negative selection promotes screening-free CRISPR-Cas9 genome editing in Caenorhabditis elegans. 33 4

Parallelized engineering of mutational models using piggyBac transposon delivery of CRISPR libraries 33 9

Optimization of multiplexed CRISPR/Cas9 system for highly efficient genome editing in Setaria viridis 33 16

CRISPR Turbo Accelerated Knock Out (CRISPy TAKO) for rapid in vivo screening of gene function 33

A mechanistic model improves off-target predictions and reveals the physical basis of SpCas9 fidelity 33 8

multicrispr: fast gRNA designer enables prime editing and parallel targeting of thousands of targets 33 4

Programmable and portable CRISPR-Cas transcriptional activation in bacteria 33 31 9

Rapid self-selecting and clone free integration of transgenes into engineered CRISPR safe harbor locations in Caenorhabditis elegans 33 18 4

High throughput functional variant screens via in-vivo production of single-stranded DNA 139 33 18 4

Pooled CRISPR screening at high sensitivity with an empirically designed sgRNA library 139 33 9

Application of CRISPR/Cas9 nuclease in amphioxus genome editing 33

Optimized sgRNA design by deep learning to balance the off-target effects and on-target activity of CRISPR/Cas9 26 4

Efficient Generation of Isogenic Primary Human Myeloid Cells using CRISPR-Cas9 Ribonucleoproteins 33 27 10 3

CRISPR/Cas9-mediated genome editing of Schistosoma mansoni acetylcholinesterase 33 10

CRISPR/Cas with ribonucleoprotein complexes and transiently selected telomere vectors allows highly efficient marker-free and multiple genome editing in Botrytis cinerea 33

Expanding the Caenorhabditis elegans auxin-inducible degron system toolkit with internal expression and degradation controls and improved modular constructs for CRISPR/Cas9-mediated genome editing 33 18

Development of a method for revertable CRISPR/Cas9-based mutagenesis in cell culture 33 18

Systematic genome-wide querying of coding and non-coding functional elements in E. coli using CRISPRi 139 33 9

Multiplex generation, tracking, and functional screening of substitution mutants using a CRISPR/retron system 139 33 18

Detecting genetic variation and base modifications together in the same single molecules of DNA and RNA at base pair resolution using a magnetic tweezer platform. 33 9 4

Parallel genetics of regulatory sequences in vivo 134 33 9

dbGuide: A database of functionally validated guide RNAs for genome editing in human and mouse cells 33 4

Native ribonucleases process sgRNA transcripts to create catalytic Cas9/sgRNA complexes in planta 33 16

Targeted mutagenesis of the Arabidopsis GROWTH-REGULATING FACTOR (GRF) gene family suggests competition of multiplexed sgRNAs for Cas9 apoprotein 33 16

Harnessing endogenous repair mechanisms for targeted gene knock-in of bovine embryos 33

Variability in mitochondrial import, mitochondrial health and mtDNA copy number using Type II and Type V CRISPR effectors 33

Predicting base editing outcomes with an attention-based deep learning algorithm trained on high-throughput target library screens 7 4

A CRISPR/Cas9-Based Approach For Editing Immortalised Human Myoblasts To Model Duchenne Muscular Dystrophy In Vitro 33 10

Parallel CRISPR-Cas9 screens clarify impacts of p53 on screen performance 139 33 9

RecT recombinase expression enables efficient gene editing in Enterococcus 33 12

Single-cell analysis of a mutant library generated using CRISPR-guided deaminase 139 43 33 9 4

CRISPR-targeted display of functional T cell receptors enables engineering of enhanced specificity and prediction of cross-reactivity 33 14 9 3

Transcriptome-wide Cas13 guide RNA design for model organisms and viral RNA pathogens 33 9 4

A versatile vector system for the fast generation of knock-in cell lines with CRISPR 33 18

SLALOM: A Simple and Rapid Method for Enzymatic Synthesis of CRISPR-Cas9 sgRNA Libraries 33 18 4

Site-specific targeting of a light activated dCas9-KIllerRed fusion protein generates transient, localized regions of oxidative DNA damage. 46 33

One-Day Construction Of Multiplex Arrays to Harness Natural CRISPR Systems 33 18 4

Development of Gene Editing Strategies for Human β-Globin (HBB) Gene Mutations 6

Automated design of CRISPR prime editors for thousands of human pathogenic variants 33 4

Novel mosaic mice with diverse applications 33 9

Detection of deleterious on-target effects after HDR-mediated CRISPR editing 33 4

Catalytically Enhanced Cas9 through Directed Protein Evolution 33 18

Discovery and engineering of small SlugCas9 with broad targeting range and high specificity and activity 33 18