Topic 171

tubulin nucleation microtubule microtubules centrosomes gamma turc xmap215 assembly regulated centrosomal turcs spindles ring centrosome nucleate spindle proteins cell biology surrounded binding phospho complexes zyg assembles reconstitution trcp plk4 localises beta organizing domain mediated leads pericentriolar bind nucleating centriolar nucleates box normal substantiating pcm centriole manner ran polymer increase minus promote segregation degradation show chromosomes mts end facilitates breakdown anaphase guanine aneuploidy mutants phosphorylates depend mutating self site assembled dtbce motif absence kinesin gtpases hydrolase strongly drosophila controls mt form pole bipolar entry promoting around function xmap215s where maintain lacks effector ends complex defects tiam1 overduplication mitotic additive expansion promotes

22 items. Top items listed below.

Phospho-regulated auto-inhibition of Cnn controls microtubule nucleation during cell division 171 68 34 3

Drosophila dTBCE recruits tubulin around chromatin to promote mitotic spindle assembly 171 68 34 8 3

Polo-like kinase 1 independently controls microtubule-nucleating capacity and size of the centrosome 171 68 34 3

Microtubule Assembly and Pole Coalescence: Early Steps in C. elegans Oocyte Meiosis I Spindle Assembly 171 68 34 8 3

XMAP215 and γ-tubulin additively promote microtubule nucleation in purified solutions 171 163 68 34 8 3

Loss of Kinesin-8 improves the robustness of the acentrosomal spindle 171 68 34 3

The transition state and regulation of γ-TuRC-mediated microtubule nucleation revealed by single molecule microscopy 171 68 34 8 3

A semi-automated machine learning-aided approach to quantitative analysis of centrosomes and microtubule organization 171 68 34 18

A stable core of GCPs 4, 5 and 6 promotes the assembly of γ-tubulin ring complexes 171 34 8 3

Biochemical reconstitutions reveal principles of human γ-TuRC assembly and function 171 8 3

Microtubules originate asymmetrically at the somatic Golgi and areguided via Kinesin-2 to maintain polarity within neurons 171 68 34 3

APC/CFZR-1 Controls ZYG-1 Levels to Regulate Centrosome Assembly 171 90 34 3

The RAC1 activator Tiam1 regulates centriole duplication through controlling PLK4 levels 171 68 34 3

Negative feedback between the APC/C and Cep152 at the centrosome regulates mitotic spindle assembly 68 34 3

LRRK2-phosphorylated Rab10 sequesters Myosin Va with RILPL2 during ciliogenesis blockade 171 102 34 3

An autonomous, but INSIG-modulated, role for the Sterol Sensing Domain in mallostery-regulated ERAD of yeast HMG-CoA reductase 171 34 8 3

Centriole-less pericentriolar material serves as a microtubule organizing center at the base of C. elegans sensory cilia 68 34 20 3

An Acentriolar Centrosome At The C. elegans Ciliary Base 68 34 20 3

Structure and reconstitution of a hydrolase complex that releases peptidoglycan from the membrane after polymerization 171 8 3

Emergent properties of HNF4α-PPARγ network may drive consequent phenotypic plasticity in NAFLD 171 38

Phytophthora sojae effector Avr1d functions as E2 competitor and inhibits ubiquitination activity of GmPUB13 to facilitate infection 171 63 3

Plexin-Bs enhance their GAP activity with a novel activation switch loop 171 63 8 3

Fungal LysM effectors that comprise two LysM domains bind chitin through intermolecular dimerization 171 8 3

Golgi-localized exo-β1,3-galactosidases involved in AGP modification and root cell expansion in Arabidopsis 171 16 3

GTP-dependent formation of straight oligomers leads to nucleation of microtubules 163 78 8 3

Cytoplasmic Microtubule Organizing Centers Regulate Meiotic Spindle Positioning in Mouse Oocyte 68 34 3

Branching microtubule nucleation is controlled by importin-mediated inhibition of TPX2 phase separation 68 34 8 3

Evidence for conformational change-induced hydrolysis of β-tubulin-GTP 68 34 8 3

Condensation of pericentrin proteins in human cells illuminates phase separation in centrosome assembly 68 34 8 3

Msps Governs Acentrosomal Microtubule Assembly and Reactivation of Quiescent Neural Stem Cells 20 3

Cdk1-dependent destabilization of long astral microtubules is required for spindle orientation 68 34 3

Plk4 triggers autonomous de novo centriole biogenesis and maturation 34 3

Confinement size determines the architecture of Ran-induced microtubule networks 68 34 8

TPX2 activation by GM130 controls astral microtubule formation and spindle orientation 68 34 20 3

Dynein-dynactin segregate meiotic chromosomes in C. elegans spermatocytes 68 34 20 3

Ana1 recruits PLK1 to mother centrioles to promote mitotic PCM assembly and centriole elongation 68 34 3

Tau avoids the GTP cap at growing microtubule plus ends 68 34 3

A novel compound that disrupts mitotic spindle poles in human cells 68 34 3

Rab14/MACF2/CAMSAP3 Complex Regulates Endosomal Targeting to the Abscission Site During Cytokinesis 68 34 3

Molecular determinants for α-tubulin methylation by SETD2 68 63 8 3

Augmin regulates kinetochore tension and spatial arrangement of spindle microtubules by nucleating bridging fibers 68 34 3

HIV-1 Gag protein with or without p6 specifically dimerizes on the viral RNA packaging signal 8 3

A release-and-capture mechanism generates an essential non-centrosomal microtubule array during tube budding 68 20 3

The insulin receptor adaptor IRS2 is an APC/C substrate that promotes cell cycle protein expression and a robust spindle assembly checkpoint 102 34 3

Spindle scaling is governed by cell boundary regulation of microtubule nucleation 68 34 8 3

Subcellular localization and mitotic interactome analyses identify SIRT4 as a centrosomally localized and microtubule associated protein 68 34 3

Microtubules form by progressively faster tubulin accretion, not by nucleation-elongation 78 8

Selective targeting of non-centrosomal AURKA functions through use of a novel targeted protein degradation tool 68 34 3

Loss of CPAP expression promotes sustained EGFR signaling and Epithelial-Mesenchymal Transition in oral cancer cells 98 14 3

Microtubule Nucleation Promoters Mto1 and Mto2 Regulate Cytokinesis in Fission Yeast 68 34 3

A conserved choreography of mRNAs at centrosomes reveals a localization mechanism involving active polysome transport 68 49 34 3

The coordinated localization of mRNA to centrosomes facilitates error-free mitosis 68 20 3

Use of the Polo-like kinase 4 (PLK4) inhibitor centrinone to investigate intracellular signaling networks using SILAC-based phosphoproteomics 102 45 3

Procentriole microtubules as drivers of centriole reduplication 68 34 3

MTCL2 is a new Golgi-resident microtubule-regulating protein, essential for organizing asymmetric microtubule network 34 8 3

Vimentin Intermediate Filaments Stabilize Dynamic Microtubules by Direct Interactions 55 8 3

Proximity labeling at non-centrosomal microtubule-organizing centers reveals VAB-10B and WDR-62 as distinct microtubule regulators 68 34 3

Microtubule re-organization during female meiosis in C. elegans 68 38 34 18

The mechanism of selective kinesin inhibition by kinesin binding protein. 51 8 3

Deubiquitylase UCHL3 drives error correction at kinetochores and chromosome segregation independent of spindle assembly checkpoint 68 34 3

Kinetochore protein Spindly controls microtubule polarity in Drosophila axons 68 34 3

Non-rodent mammalian zygotes assemble dual spindles despite the presence of paternal centrosomes 68 34 20

Mask, the Drosophila Ankyrin Repeat and KH domain-containing protein, regulates microtubule dynamics 34 3

Reconstitution of human kinetochore in mitotic cell extracts reveals permitted and restricted assembly steps 68 34 8 3

DIAPH3 deficiency links microtubules to mitotic errors, defective neurogenesis, and brain dysfunction 68 34 3

Critical role for cold shock protein YB-1 in cytokinesis 102 34 3

Microtubules tune mechanosensitive cell responses 55 34 3

Autoregulatory control of microtubule binding in the oncogene, doublecortin-like kinase 1 102 63 8 3

Stable kinetochore-microtubule attachments restrict MTOC position and spindle elongation in acentrosomal oocytes 68 34 8 3

Competitive Microtubule Binding of PEX14 Coordinates Peroxisomal Protein Import and Motility 63 8 3

Nucleotide binding, evolutionary insights and interaction partners of the pseudokinase Unc-51-like kinase 4 63 8 3

Gfral-expressing Neurons Suppress Food Intake via Aversive Pathways 171 11 10 3

Ndel1 promotes keratin assembly and desmosome stability 34 3

Orderly assembly underpinning built-in asymmetry in the yeast centrosome duplication cycle requires cyclin-dependent kinase 68 34 20 3

Emergent properties of a mitotic Kif18b-MCAK-EB network 68 34 8 3

Spatial Variation of Microtubule Depolymerization in Large Asters Suggests Regulation by MAP Depletion 7

Single depolymerizing and transport kinesins stabilize microtubule ends 127 55 8 3

Two modes of PRC1-mediated mechanical resistance to kinesin-driven microtubule network disruption 68 55 8 3

A non-canonical function for Centromere-associated protein-E (CENP-E)controls centrosome integrity and orientation of cell division 68 34 3

Plasmodium berghei kinesin-5 associates with the spindle apparatus during cell division and is important for efficient production of infectious sporozoites 68 60 34 3

K-fiber bundles in the mitotic spindle are mechanically reinforced by Kif15 68 34 8 3

The association of Plk1 with the Astrin-Kinastrin complex promotes formation and maintenance of a metaphase plate 68 7 3

Ancient MAPK ERK7 is regulated by an unusual inhibitory scaffold required for Toxoplasma apical complex biogenesis 34 8 3

Heh2/Man1 may be an evolutionarily conserved sensor of NPC assembly state 151 34 8 3

The proteomic landscape of centromeric chromatin reveals an essential role for the Ctf19CCAN complex in meiotic kinetochore assembly 68 34 3

Klp2 and Ase1 synergize to maintain meiotic spindle stability during metaphase I 68 34 3

Nano-positioning and tubuline conformation determine transport of mitochondria along microtubules 34 8 3

Intrinsically disordered domain of kinesin-3 Kif14 enables unique functional diversity 68 8 3

Differential Regulation of Single Microtubules and Cross-linked Bundles by a Minimal Three-Protein Module 68 34 8 3

Coordinated crosstalk between microtubules and actin by a spectraplakin regulates lumen formation and branching 55 34 20 3

UNC-45A Destabilizes MTs Independent of its Effect on Non-Muscle Myosin II 34 3

Cep57 and Cep57L1 cooperatively maintain centriole engagement during interphase to ensure proper centriole duplication cycle 68 34 3

Exportin Crm1 is important for Swi6 nucleocytoplasmic shuttling and MBF transcription activation in Saccharomyces cerevisiae 34 3

Centrosome and ciliary abnormalities in fetal akinesia deformation sequence human fibroblasts 34 3

Roles of motor on-rate and cargo mobility in intracellular transport 127 8

Abelson-induced phosphorylation of TACC3 modulates its interaction with microtubules and affects its impact on axon outgrowth and guidance 102 34 3

Parkinson's Disease-linked LRRK2 structure and model for microtubule interaction 51 8 3

Structure of MlaFB uncovers novel mechanisms of ABC transporter regulation 34 8 3

Optical flow analysis reveals that Kinesin-mediated advection impacts on the orientation of microtubules in the Drosophila oocyte 55 34 3

Modeling disease-correlated TUBA1A mutation in budding yeast reveals a molecular basis for tubulin dysfunction 68 34 3