Topic 68

spindle mitotic microtubule mitosis microtubules segregation chromosome kinetochore division anaphase cell chromosomes centrosomes biology during assembly metaphase centrosome kinetochores proper spindles interphase tubulin meiosis kinesin kinase yeast nucleation cycle localization meiotic centrosomal pole aneuploidy cytokinesis attachments defects daughter regulated phosphorylation end poles proteins functions promotes recruitment checkpoint aurora protein polo exit required centromeres positioning divisions budding arrest forces astral cells dynein errors formation essential attachment sister ends minus chromatids defective dependent oocytes centromeric promote localizes pericentriolar chromosomal centrioles nuclear plk1 cdk1 regulates motors mt fission binding depletion chromatin propose centriole regulate at depolymerization mts requires organization maintain asymmetric orientation pp2a

169 items. Top items listed below.

Deubiquitylase UCHL3 drives error correction at kinetochores and chromosome segregation independent of spindle assembly checkpoint 68 34 3

The association of Plk1 with the Astrin-Kinastrin complex promotes formation and maintenance of a metaphase plate 68 7 3

Cdk1-dependent destabilization of long astral microtubules is required for spindle orientation 68 34 3

Kinetochore-independent sister chromosome separation is driven by plus-end microtubule dynamics 68 34 3

Klp2 and Ase1 synergize to maintain meiotic spindle stability during metaphase I 68 34 3

Drosophila dTBCE recruits tubulin around chromatin to promote mitotic spindle assembly 171 68 34 8 3

Negative feedback between the APC/C and Cep152 at the centrosome regulates mitotic spindle assembly 68 34 3

The proteomic landscape of centromeric chromatin reveals an essential role for the Ctf19CCAN complex in meiotic kinetochore assembly 68 34 3

The chromosomal passenger complex establishes chromosome biorientation via two parallel localization pathways 68 34 3

Augmin regulates kinetochore tension and spatial arrangement of spindle microtubules by nucleating bridging fibers 68 34 3

Plasmodium berghei kinesin-5 associates with the spindle apparatus during cell division and is important for efficient production of infectious sporozoites 68 60 34 3

The C-terminal helix of BubR1 is essential for CENP-E-dependent chromosome alignment 68 34 8 3

Dynein-dynactin segregate meiotic chromosomes in C. elegans spermatocytes 68 34 20 3

TPX2 activation by GM130 controls astral microtubule formation and spindle orientation 68 34 20 3

Centriole-less pericentriolar material serves as a microtubule organizing center at the base of C. elegans sensory cilia 68 34 20 3

Microtubule Assembly and Pole Coalescence: Early Steps in C. elegans Oocyte Meiosis I Spindle Assembly 171 68 34 8 3

Aurora B and Condensin are dispensable for chromosome arm and telomere separation during meiosis II 68 34 3

The myosin regulatory light chain Myl5 localizes to mitotic spindle poles and is required for proper cell division 68 34 3

Tension promotes kinetochore-microtubule release in response to Aurora B activity 68 34 3

Microtubule poleward flux in human cells is driven by the coordinated action of four kinesins 68 34 3

Polo-like kinase 1 independently controls microtubule-nucleating capacity and size of the centrosome 171 68 34 3

Loss of Kinesin-8 improves the robustness of the acentrosomal spindle 171 68 34 3

K-fiber bundles in the mitotic spindle are mechanically reinforced by Kif15 68 34 8 3

Mechanisms of MTOC clustering and spindle positioning in the budding yeast Cryptococcus neoformans: a microtubule grow-and-catch model 68 34 8

NuMA interaction with chromatin is vital for proper nuclear architecture in human cells 68 34 3

chTOG is a conserved mitotic error correction factor 68 34 3

Ran-GTP is non-essential to activate NuMA for spindle pole focusing, but dynamically polarizes HURP to control mitotic spindle length 68 34 3

MTFR2 regulates mitochondrial fission and impacts spindle integrity during mitosis 68 34 3

PP2A:B56 Regulates Meiotic Chromosome Segregation in C. elegans Oocytes 68 34 3

Modeling Reveals Cortical Dynein-Dependent Oscillations in Bipolar Spindle Length 68 38 34 8

Confinement size determines the architecture of Ran-induced microtubule networks 68 34 8

Evidence that polyploidy in esophageal adenocarcinoma originates from mitotic slippage caused by defective chromosome attachments 68 9 3

The coordination of spindle-positioning forces during the asymmetric division of the C. elegans zygote is revealed by distinct microtubule dynamics at the cortex. 68 34 8

The RAD51 recombinase protects mitotic chromatin in human cells 68 46 34 3

A non-canonical function for Centromere-associated protein-E (CENP-E)controls centrosome integrity and orientation of cell division 68 34 3

Shugoshin promotes efficient activation of spindle assembly checkpoint and timely spindle disassembly 68 34 3

Mps1 promotes poleward chromosome movements in meiotic pro-metaphase 68 34 3

The collapse of the spindle following ablation in S. pombe is mediated by microtubules and the motor protein dynein 68 55 34 8

Rab14/MACF2/CAMSAP3 Complex Regulates Endosomal Targeting to the Abscission Site During Cytokinesis 68 34 3

Emergent properties of a mitotic Kif18b-MCAK-EB network 68 34 8 3

The structural basis for kinetochore stabilization by Cnn1/CENP-T 68 34 8 3

The mitotic protein NuMA plays a spindle-independent role in nuclear formation and mechanics 68 34 8 3

Optogenetic control of PRC1 reveals that bridging fibers promote chromosome alignment by overlap length-dependent forces 68 34 3

Metazoan-like kinetochore arrangement masked by the interphase RabI configuration 68 34 8 3

Kinetochore protein Spindly controls microtubule polarity in Drosophila axons 68 34 3

DUSP7 Regulates the Activity of ERK2 to PromoteProper Chromosome Alignment During Cell Division 68 34 3

Selective vulnerability of aneuploid human cancer cells to inhibition of the spindle assembly checkpoint 68 14 3

Cytoplasmic Microtubule Organizing Centers Regulate Meiotic Spindle Positioning in Mouse Oocyte 68 34 3

Chromosome-directed oocyte spindle assembly depends HP1 and the Chromosomal Passenger Complex 68 34 8 3

Reconstitution of human kinetochore in mitotic cell extracts reveals permitted and restricted assembly steps 68 34 8 3

CDK1 controls CHMP7-dependent nuclear envelope reformation 68 34 3

Whole genome doubling confers unique genetic vulnerabilities on tumor cells 68 14 3

Chromosomally unstable tumor cells specifically require KIF18A for proliferation 68 14 3

Spindle position dictates division site during asymmetric cell division in moss 68 34 3

Spindle scaling is governed by cell boundary regulation of microtubule nucleation 68 34 8 3

Condensation of pericentrin proteins in human cells illuminates phase separation in centrosome assembly 68 34 8 3

Transcription factor Sp1 regulates mitotic fidelity through Aurora B kinase-mediated condensin I localization 68 34 3

Branching microtubule nucleation is controlled by importin-mediated inhibition of TPX2 phase separation 68 34 8 3

PP2A-Cdc55 is responsible for mitotic arrest in DNA re-replicating cells in S. cerevisiae 68 46 34 3

Gravin-associated kinase signaling networks coordinate γ-tubulin organization at mitotic spindle poles 68 34 3

Protein Phosphatase 1 in association with Bud14 inhibits mitotic exit in Saccharomyces cerevisiae 68 34 3

Mms19 promotes spindle microtubule assembly in neural stem cells through two distinct pathways 68 34 20 3

DIAPH3 deficiency links microtubules to mitotic errors, defective neurogenesis, and brain dysfunction 68 34 3

Kinetochore individualization in meiosis I is required for centromeric cohesin removal in meiosis II 68 34 8

Evidence for conformational change-induced hydrolysis of β-tubulin-GTP 68 34 8 3

An Acentriolar Centrosome At The C. elegans Ciliary Base 68 34 20 3

Real-time dynamics of Plasmodium NDC80 reveals unusual modes of chromosome segregation during parasite proliferation 68 60 34 8 3

Length regulation of microtubule antiparallel overlaps by molecular motors 68 8 3

An unbiased, quantitative and versatile method for determining misaligned and lagging chromosome during mitosis 68 34 18

Peripheral microtubules ensure asymmetric furrow positioning in neural stem cells 68 34 20

Modeling disease-correlated TUBA1A mutation in budding yeast reveals a molecular basis for tubulin dysfunction 68 34 3

Dynein collective behavior in mitotic nuclear positioning of Saccharomyces cerevisiae 68 34 8

Stable kinetochore-microtubule attachments restrict MTOC position and spindle elongation in acentrosomal oocytes 68 34 8 3

Subcellular localization and mitotic interactome analyses identify SIRT4 as a centrosomally localized and microtubule associated protein 68 34 3

Microtubule attachment and centromeric tension shape the protein architecture of the human kinetochore 68 34 8 3

A semi-automated machine learning-aided approach to quantitative analysis of centrosomes and microtubule organization 171 68 34 18

Non-rodent mammalian zygotes assemble dual spindles despite the presence of paternal centrosomes 68 34 20

The MPS1 kinase NTE region has helical propensity and preferred conformations towards the TPR domain 68 8

Dissection of the Spindle Assembly Checkpoint by Proximity Proteomics 68 34 3

C. elegans CLASP/CLS-2 negatively regulates membrane ingression throughout the oocyte cortex and is required for polar body extrusion 68 34 20 3

Two modes of PRC1-mediated mechanical resistance to kinesin-driven microtubule network disruption 68 55 8 3

Lamin C regulates genome organization after mitosis 89 68 34 20 3

Multiple pools of Protein Phosphatase 2A-B56 function to antagonize spindle assembly, promote kinetochore attachments and maintain cohesion in Drosophila Oocytes 68 34 3

Roles of developmentally regulated KIF2A alternative isoforms in cortical neuron migration and differentiation 133 68 20 11 3

Functional analysis of the chromosomal passenger complex in Arabidopsis 68 34 16 3

Defining endogenous TACC3-chTOG-clathrin-GTSE1 interactions at the mitotic spindle using induced relocalization 7 3

A novel compound that disrupts mitotic spindle poles in human cells 68 34 3

Initial spindle formation at the oocyte center protects against incorrect kinetochore-microtubule attachment and aneuploidy in mice 68 34 15

Excess crossovers impede faithful meiotic chromosome segregation in C. elegans 68 46 34

Identification and characterization of centromeric sequences in Xenopus laevis 68 31 9

The insulin receptor adaptor IRS2 is an APC/C substrate that promotes cell cycle protein expression and a robust spindle assembly checkpoint 102 34 3

Selective targeting of non-centrosomal AURKA functions through use of a novel targeted protein degradation tool 68 34 3

PRC1 and EB1 Binding Dynamics Reveal a Solidifying Central Spindle during Anaphase Compaction in Human Cells 68 34 8 3

Parental genome unification is highly erroneous in mammalian embryos 68 20

Molecular determinants for α-tubulin methylation by SETD2 68 63 8 3

The coordinated localization of mRNA to centrosomes facilitates error-free mitosis 68 20 3

THE SPATIAL REGULATION OF CONDENSIN ACTIVITY IN CHROMOSOME CONDENSATION 68 46 34 8 3

The First Mitotic Division of the Human Embryo is Highly Error-prone 68 46 34 20

The conserved Cdc14 phosphatase ensures effective resolution of meiotic recombination intermediates. 46 34 3

Ase1 domains dynamically slow anaphase spindle elongation and recruit Bim1 to the midzone 68 34 8 3