Topic 125
sequencing samples taxonomic sample method microbial amplicon reads throughput 16s metabarcoding dna communities library abundance community rrna mock taxa metagenomic primers microbiome libraries methods profiling environmental sequences cost high amplification composition identification species pcr pipeline illumina ribosomal protocol approach shotgun detection sequence efficient metagenomics used reference accurate based preparation workflow read sequenced primer organisms biodiversity available kits applied resolution present generation edna commercially genomics assessment bias biases microbiology bacterial low characterization microbiomes provides challenges tool rna effective datasets alternative abundances amplicons diversity microbes 18s widely next approaches reliable steps extraction monitoring using construction enables relative from technique bacteria commonly miseq
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