Topic 35
detection pcr diagnostic rt samples assay cov sars covid 19 testing qpcr diagnosis sensitivity amplification pandemic rapid detect assays viral extraction rna sample nasopharyngeal virus coronavirus clinical test tests diagnostics reaction nucleic reverse swabs real limit microbiology method reagents detecting primers isothermal specificity sensitive molecular copies 100 positive swab laboratories laboratory primer polymerase need developed respiratory 2019 time lamp protocol standard cost kits care disease dna tested cdc outbreak minutes quantitative chain simple screening methods worldwide specimens low detected probe negative step gold commercial per health performance acute surveillance throughput current syndrome currently based spread using equipment saliva accurate ct
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