Topic 35

detection pcr diagnostic rt samples assay cov sars covid 19 testing qpcr diagnosis sensitivity amplification pandemic rapid detect assays viral extraction rna sample nasopharyngeal virus coronavirus clinical test tests diagnostics reaction nucleic reverse swabs real limit microbiology method reagents detecting primers isothermal specificity sensitive molecular copies 100 positive swab laboratories laboratory primer polymerase need developed respiratory 2019 time lamp protocol standard cost kits care disease dna tested cdc outbreak minutes quantitative chain simple screening methods worldwide specimens low detected probe negative step gold commercial per health performance acute surveillance throughput current syndrome currently based spread using equipment saliva accurate ct

230 items. Top items listed below.

Robust and sensitive detection of SARS-CoV-2 using PCR based methods 35 1

Rapid direct nucleic acid amplification test without RNA extraction for SARS-CoV-2 using a portable PCR thermocycler 35 1

Development of Reverse Transcription Loop-mediated Isothermal Amplification (RT-LAMP) Assays Targeting SARS-CoV-2 35 1

A Combined approach of MALDI-TOF Mass Spectrometry and multivariate analysis as a potential tool for the detection of SARS-CoV-2 virus in nasopharyngeal swabs. 35 1

Rapid Isothermal Amplification and Portable Detection System for SARS-CoV-2 35 1

Evaluation of Different PCR Assay Formats for Sensitive and Specific Detection of SARS-CoV-2 RNA 35 1

Pooling nasopharyngeal swab specimens to increase testing capacity for SARS-CoV-2 35 1

Optimized qRT-PCR approach for the detection of intra- and extracellular SARS-CoV-2 RNAs 35 1

An enhanced isothermal amplification assay for viral detection 35 1

The Potential Use of Unprocessed Sample for RT-qPCR Detection of COVID-19 without an RNA Extraction Step 35 1

A comparative study of isothermal nucleic acid amplification methods for SARS-CoV-2 detection at point of care 35 1

One-step RNA extraction for RT-qPCR detection of 2019-nCoV 35 1


An Inexpensive RT-PCR Endpoint Diagnostic Assay for SARS-CoV-2 Using Nested PCR: Direct Assessment of Detection Efficiency of RT-qPCR Tests and Suitability for Surveillance 35 1

A simple RNA preparation method for SARS-CoV-2 detection by RT-qPCR 35 1

Evaluation Of SYBR Green Real Time PCR For Detecting SARS-CoV-2 From Clinical Samples 35 1

A simple, safe and sensitive method for SARS-CoV-2 inactivation and RNA extraction for RT-qPCR 35 1

Extraction-free COVID-19 (SARS-CoV-2) diagnosis by RT-PCR to increase capacity for national testing programmes during a pandemic 35 1

Development of RNA-based assay for rapid detection of SARS-CoV-2 in clinical samples 35 1

Design of a Novel Multiplex Real Time RT-PCR Assay for SARS-CoV-2 Detection 35 1

A Highly Scalable and Rapidly Deployable RNA Extraction-Free COVID-19 Assay by Quantitative Sanger Sequencing 35 1

Rapid detection of SARS-CoV-2 and other respiratory viruses by using LAMP method with Nanopore Flongle workflow 35 1

Development Optimization and Validation of RT-LAMP based COVID-19 Facility in Pakistan 35 1

A Rapid COVID-19 RT-PCR Detection Assay for Low Resource Settings 35 1

SARS-CoV2 Testing: The Limit of Detection Matters 35 1

All-in-One Dual CRISPR-Cas12a (AIOD-CRISPR) Assay: A Case for Rapid, Ultrasensitive and Visual Detection of Novel Coronavirus SARS-CoV-2 and HIV virus 35 1

A rapid, highly sensitive and open-access SARS-CoV-2 detection assay for laboratory and home testing 35 1

Comparison of commercial RT-PCR diagnostic kits for COVID-19 35 1


A rapid, low cost, and highly sensitive SARS-CoV-2 diagnostic based on whole genome sequencing 35 1

Highly sensitive and full-genome interrogation of SARS-CoV-2 using multiplexed PCR enrichment followed by next-generation sequencing 35 1

Analytical Validation of a COVID-19 qRT-PCR Detection Assay Using a 384-well Format and Three Extraction Methods 35 1

Pre-treatment of the clinical sample with Proteinase K allows detection of SARS-CoV-2 in the absence of RNA extraction 35 1

Validation of a Single-step, Single-tube Reverse Transcription-Loop-Mediated Isothermal Amplification Assay for Rapid Detection of SARS-CoV-2 RNA 35 1

SARS-CoV-2 detection from nasopharyngeal swab samples without RNA extraction 35 1

Comparative performance of four nucleic acid amplification tests for SARS-CoV-2 virus 35 1

Evaluation of commercial qPCR kits for detection of SARS-CoV-2 in pooled samples 35 1

Direct colorimetric LAMP assay for in-field detection of African swine fever virus: a validation study during an outbreak in Vietnam 35 15

Nanotrap(R) particles improve detection of SARS-CoV-2 for pooled sample methods, extraction-free saliva methods, and extraction-free medium methods 35 1

Chimeric synthetic reference standards enable cross-validation of positive and negative controls in SARS-CoV-2 molecular tests. 35 1

Analytical and Clinical Comparison of Three Nucleic Acid Amplification Tests for SARS-CoV-2 Detection 35 1

REMBRANDT: A high-throughput barcoded sequencing approach for COVID-19 screening. 35 1

SARS-CoV-2 detection with CRISPR diagnostics 103 35 1

Enhancing Colorimetric LAMP Amplification Speed and Sensitivity with Guanidine Chloride 35 18 1

Assessment of the use and quick preparation of saliva for rapid microbiological diagnosis of COVID-19. 35 15 1

Comparative analyses of SARS-CoV-2 binding (IgG, IgM, IgA) and neutralizing antibodies from human serum samples 130 24 1

Evaluating the efficacy of RT-qPCR SARS-CoV-2 direct approaches in comparison to RNA extraction 35 1

Resource optimization in COVID-19 diagnosis 35 1

Validation and Comparison of a Modified CDC Assay with two Commercially Available Assays for the Detection of SARS-CoV-2 in Respiratory Specimen 35 1

Performance of Abbott ID NOW COVID-19 rapid nucleic acid amplification test in nasopharyngeal swabs transported in viral media and dry nasal swabs, in a New York City academic institution 35 1

Comparison of three TaqMan Real-Time Reverse Transcription-PCR assays in detecting SARS-CoV-2 35 7 1

Improved and Simplified Diagnosis of Covid-19 using TE Extraction from Dry Swabs 35 1

Pitfalls in SARS-CoV-2 PCR diagnostics 35 1

Head-to-head comparison of four antigen-based rapid detection tests for the diagnosis of SARS-CoV-2 in respiratory samples 35 15 1

Comparison of SARS-CoV-2 Indirect and Direct Detection Methods 35 1

Recommendations for sample pooling on the Cepheid GeneXpert(R) system using the Cepheid Xpert(R) Xpress SARS-CoV-2 assay 35 1

Preliminary support for a 'dry swab, extraction free' protocol for SARS-CoV-2 testing via RT-qPCR 35 1

Integrated sample inactivation, amplification, and Cas13-based detection of SARS-CoV-2 35 1

CoV2ID: Detection and Therapeutics Oligo Database for SARS-CoV-2 35 1

Programmable low-cost DNA-based platform for viral RNA detection 35 1

SYBR green one-step qRT-PCR for the detection of SARS-CoV-2 RNA in saliva 35 1

Analysis of the potential impact of genomic variants in SARS-CoV-2 genomes from India on molecular diagnostic assays 37 35 1

In silico prediction of COVID-19 test efficiency with DinoKnot 35 1

A CRISPR-based SARS-CoV-2 diagnostic assay that is robust against viral evolution and RNA editing 35 7 1

Role of Anti-SARS-CoV-2 antibodies in different cohorts: Can they provide clues for appropriate patient triaging? 130 35 1

Evaluation of the performance of SARS-CoV-2 serological tools and their positioning in COVID-19 diagnostic strategies. 130 35 1

Overhauling a faulty control in the CDC-recommended SARS-CoV-2 RT-PCR test panel 35 1

Massive Multiplexing Can Deliver a $1 Test for COVID-19 35 1

One-pot Detection of COVID-19 with Real-time Reverse-transcription Loop-mediated Isothermal Amplification (RT-LAMP) Assay and Visual RT-LAMP Assay 35 1

Comparison of 12 molecular detection assays for SARS-CoV-2 35 15 1

Electric-field-driven microfluidics for rapid CRISPR-based diagnostics and its application to detection of SARS-CoV-2 35 18 4 1

The Theory and Practice of the viral dose in neutralization assay: insights on SARS-CoV-2 "doublethink" effect. 35 1

Proteotyping SARS-CoV-2 virus from nasopharyngeal swabs: a proof-of-concept focused on a 3 min mass spectrometry window 35 1

An ultrasensitive, rapid, and portable coronavirus SARS-CoV-2 sequence detection method based on CRISPR-Cas12 103 35 1

High throughput detection and genetic epidemiology of SARS-CoV-2 using COVIDSeq next generation sequencing 35 1

High speed large scale automated isolation of SARS-CoV-2 from clinical samples using miniaturized co-culture coupled with high content screening 35 1

Patient DNA cross-reactivity of the CDC SARS-CoV-2 extraction control leads to an inherent potential for false negative results 35 1

Rapid and quantitative detection of COVID-19 markers in micro-liter sized samples 130 35 1

Efficacy of a novel SARS-CoV-2 detection kit without RNA extraction and purification 35 1

Reverse-transcription recombinase-aided amplification assay for H5 subtype avian influenza virus 35 15

Highly multiplexed oligonucleotide probe-ligation testing enables efficient extraction-free SARS-CoV-2 detection and viral genotyping 35 1

A Fast and Accessible Method for the Isolation of RNA, DNA, and Protein to Facilitate the Detection of SARS-CoV-2 35 1

A simple magnetic nanoparticles-based viral RNA extraction method for efficient detection of SARS-CoV-2 35 1

Comparison of Cepheid Xpert Xpress and Abbott ID Now to Roche cobas for the Rapid Detection of SARS-CoV-2 35 15 1

Real-time colorimetric LAMP methodology for quantitative nucleic acids detection at the point-of-care 35 18 4 1

HiDRA-seq: High-Throughput SARS-CoV-2 Detection by RNA Barcoding and Amplicon Sequencing 35 1

Saliva-Based Molecular Testing for SARS-CoV-2 that Bypasses RNA Extraction 35 1

Development and evaluation of a loop-mediated isothermal amplification (LAMP) assay for the detection of Tomato brown rugose fruit virus (ToBRFV) 35 15

Detection of SARS-CoV-2 RNA by multiplex RT-qPCR 35 1

Multiplex recombinase polymerase amplification (RPA) assay developed using unique genomic regions and coupled with a lateral flow device for rapid on-site detection of genus Clavibacter and C. nebraskensis 35 12

Validation and Performance Comparison of Three SARS-CoV-2 Antibody Assays 130 35 1

BioLaboro: A bioinformatics system for detecting molecular assay signature erosion and designing new assays in response to emerging and reemerging pathogens 35 1

Ultra-sensitive nanozyme-based chemiluminescence paper test for rapid diagnosis of SARS-CoV-2 infection 35 1

Dog Savior: Immediate Scent-Detection of SARS-COV-2 by Trained Dogs 35 1

INSIGHT: a population scale COVID-19 testing strategy combining point-of-care diagnosis with centralised high-throughput sequencing 35 18 4

Saliva sampling is an excellent option to increase the number of SARS CoV2 diagnostic tests in settings with supply shortages 35 1

Mass Spectrometric detection of SARS-CoV-2 virus in scrapings of the epithelium of the nasopharynx of infected patients via Nucleocapsid N protein 35 1

Clinical performance of SARS-CoV-2 IgG antibody tests and potential protective immunity 130 35 1

Evaluation of the EUROIMMUN Anti-SARS-CoV-2 ELISA Assay for detection of IgA and IgG antibodies 130 35 1

Optimization of SARS-CoV-2 detection by RT-QPCR without RNA extraction 35 1